ABSTRACT
Objective@#To identify the potential categories of alexithymia in college students, and further analyze the relationship between different potential categories and social anxiety and depression, so as to provide theoretical basis for promoting the development of positive emotions and mental health of college.@*Methods@#A cross sectional survey was conducted among 1 338 students randomly selected from a cluster of four universities in Hebei, Jilin, Liaoning Provinces in September 2020 by convenience sampling method. Chinese Version of Twenty item Toronto Alexithymia Scale, Social Interaction Anxiety Scale, Center for Epidemiologic Studies Depression were used for survey, potential profile analysis and Logistic regression analysis were performed on the data.@*Results@#College students with alexithymia could be divided into three potential categories:good emotion expression group( 33.3 %), good emotion discrimination group(9.6%), and difficulty in emotion expression group(57.1%). Logistic regression analysis showed that after controlling for demographic variables, the group with good emotional expression was negatively correlated with depression symptoms ( β =-1.92,-1.44, P <0.01), and social anxiety ( β =-0.71,-2.04, P <0.01).@*Conclusion@#Alexithymia of college students shows a category distribution, and each potential category of alexithymia has different associations with social anxiety and depression symptoms. To improve the mental health, teachers in colleges and universities in the mental health education work to develop targeted training of emotional regulation ability of college students to formulate intervention programs, relieve the symptoms of social anxiety and depression of college students, promote their positive psychological development.
ABSTRACT
@# Objective: To investigate the effect of C-phycocyanin (C-PC) on the epithelial-mesenchymal transition (EMT) of cervical cancer Caski cells induced by transforming growth factor beta1 (TGF-β1). Methods: According to different treatment methods, Caski cells were divided into three groups: 10 ng/ml TGF-β1 treatment group, 10 ng/ml TGF-β1+300 μg/ml C-PC co-treatment group and control group (untreated). After 24 h of treatment, the morphological changes of Caski cells were observed, and the effects of TGF-β1 and C-PC on the migration and invasion of Caski cells were detected by Scratch test and Transwell test, respectively. Western blotting was used to detect the effect of C-PC on the expression of epithelial phenotypic marker protein E-cadherin and stromal phenotypic marker protein N-cadherin in TGF-β1-induced Caski cells, and qPCR was used to detect the mRNA expressions of EMT related factors Snail, Zeb1 and Twist. Results: Caski cells in the TGF-β1 treatment group lost the characteristics of the original epithelial phenotype, while the cells in the TGF-β1+C-PC co-treatment group maintained the characteristics of normal epithelial phenotype; the migration rate ([60.0±1.4]% vs [33.5±2.2]%, [40.0±2.8]%, both P<0.05) and the number of invasive transmembrane cells ([108.2±6.2] vs [25.2±3.1], [39.8±5.4], both P<0.01]) of Caski cells in the TGF- β1 treatment group were significantly higher than those in the co-treatment group and the control group. Compared with the control group, the expression of E-cadherin in Caski cells treated with TGF-β1 decreased significantly (P<0.05), while the mRNA expressions of Twist, Snail and Zeb1 increased significantly (all P<0.05); However, co-treatment with C-PC reversed above changes (P<0.05 or P<0.01), and significantly decreased the protein expression level of N-cadherin (P< 0.05). Conclusion: C-PC treatment can inhibit the invasion and metastasis ability of Caski cells induced by TGF-β1 and further affects the EMT process. The mechanism may be related to the decrease of mRNAexpressions of Twist, Snail and Zeb1 by C-PC treatment. ·